Distribution of dimethylarginine-dimethylaminohydrolase-II (DDAH2) gene polymorphism in hemodialysis patients.

Thaha, M. ,

Yusuf, M.,

Empitu, M.A.,

Bakarman, A.,

Tomino, Y.

Department of Internal Medicine, Faculty of Medicine, Airlangga University, Surabaya, Indonesia. 

Abstract

to describe the distribution of single nucleotide polymorphisms (SNPs) at -449 (promoter region) DDAH 2 in hemodialysis (HD) patients. this study was a descriptive study, 56 HD patients and 30 healthy individuals were enrolled. Based on its etiology, the HD patient group was further divided into hypertension (HT) group and non-HT group. DNA was extracted from whole blood samples with a commercially available DNA isolation kit. Genotyping of the polymorphisms was performed using PCR-based SNP detection methods (Applied Biosystems, Carlsbad, USA) based on 5'-exonuclease activity assays for rs805305 (-449 G/C). Allelic variation was assessed in each participant. heterozygotes were observed as the most abundant genotypes in both groups (70% in healthy individuals and 55% in HD patients), followed by GG genotype in the HD patients (30%), while CC (27%) was the second most common genotype polymorphism in the healthy individuals. there is a significant difference in distribution of DDAH 2 gene polymorphism among HD patients compared to healthy individuals (p=0.01).

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